If you're looking for a good learning tool that explains the effect of different parameters on an HPLC separation then it might be worth taking a look at HPLC Simulator.
It is described as follows on the developer's website:
"HPLC Simulator
is a web-based high-performance liquid chromatography simulation. Adjust
a range of experimental parameters and see their affect on
chromatographic parameters including retention time, column efficiency,
and backpressure. It is intended to be used for educational purposes
only."
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