When delivering HPLC method development training, I usually advise that the pKa of an analyte is taken into consideration, if appropriate, when selecting a suitable buffer pH. The problem with this approach is that the pKa value is not always known. A resource which may prove helpful is provided in the Chemistry Resources section of the Chemagination website where there are directions on ‘How to measure pKa by UV-vis spectrophotometry’.
Hi. My name is Oona McPolin and my company is called Mourne Training Services Ltd. I am a trainer, consultant and auditor specialising in chemical analysis of pharmaceuticals and biopharmaceuticals. This blog has now retired. You can visit the new blog on the MTS website