When delivering HPLC method development training, I usually advise that the pKa of an analyte is taken into consideration, if appropriate, when selecting a suitable buffer pH. The problem with this approach is that the pKa value is not always known. A resource which may prove helpful is provided in the Chemistry Resources section of the Chemagination website where there are directions on ‘How to measure pKa by UV-vis spectrophotometry’.
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