MTS Recommends... Learning Tool for HPLC

If you're looking for a good learning tool that explains the effect of different parameters on an HPLC separation then it might be worth taking a look at HPLC Simulator.

It is described as follows on the developer's website:

"HPLC Simulator is a web-based high-performance liquid chromatography simulation. Adjust a range of experimental parameters and see their affect on chromatographic parameters including retention time, column efficiency, and backpressure. It is intended to be used for educational purposes only."