How Reversed-Phase Liquid Chromatography Works
By: Mark R. Schure, Jake L. Rafferty, Ling Zhang, J. Ilja Siepmann
LCGC North America
Volume 31, Issue 8
Aug 1, 2013
Described as follows by the authors:
"The keys to understanding reversed-phase liquid chromatography (LC) are provided at the molecular mechanism level as determined by high accuracy molecular simulation. The essential features of C 18 stationary-phase chains in contact with methanol–water and acetonitrile–water mixtures are discussed in the context of bonded-chain geometry, spatial distribution of alkane and alcohol solutes, retention mechanism, and retention thermodynamics. This tutorial is intended to be applicable to a wide audience ranging from occasional users of liquid chromatography to separation scientists."
MTS verdict: An interesting read for any HPLC method developer.