The Use of Temperature for Method Development in LC by Gerd Vanhoenacker , Frank David & Pat Sandra, Chromatography Today, September 2010
Since the ionisation equilibria of analytes and mobile phase for polar and ionisable analytes is temperature dependent, temperature can be used as a useful selectivity parameter when developing methods for these types of analytes. This technical note discusses the range of temperatures which can be used for method development and the different types of available LC columns which can be used at high temperatures.
Thursday, 20 January 2011
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