Friday, 20 March 2020

COVID-19: Business as Usual for MTS Consultancy Services

Mourne Training Services would like to assure our customers that our consultancy services will continue as usual during these difficult times of dealing with the effects of  the coronavirus (SARS-CoV-2) causing COVID-19. Obviously, travel to your site is no longer an option but we will continue to offer remote review of your issues and provide expert advice.

We can advise on all aspects of the chemical analysis of pharmaceuticals and biopharmaceuticals. Examples of our remote review service has recently included the review of:
  • Out of trend (OOT) and out of specification (OOS) investigations;
  • Analytical methodology for analysis of nitrosamines by LC-MS/MS
  • Method validation protocol
  • Pharmacopoeia method verification protocol
  • SOP on method validation
  • Analytical method data for submission of a variation
There are more examples on the MTS website, click here.

If this service would be of interest then please contact us to find out how we can help.

COVID-19: Update to MTS Training Schedule 2020

Alongside the most serious health and welfare effects of  the coronavirus (SARS-CoV-2) causing COVID-19 are those relating to businesses and the inability to travel. Regarding the training provided by Mourne Training Services, we have decided to introduce a live training version of our classroom based courses and cancel our open enrolment courses that were planned for London and Berlin in June 2020.

So if you would like to attend our courses from your home or workplace then you will just need a device with an internet connection to access our virtual environment for learning, e-MTS. The courses will run over 3 days in the same way that the open enrolment courses do, so it will suit learners who prefer to have a fixed time scheduled for training and can attend for the full number of days required.

Currently this only applies to the courses, 'Validation & Transfer of Methods for Biopharmaceutical Analysis', and, 'Validation & Transfer of Methods for Pharmaceutical Analysis', but depending on uptake, we may extend this option to other courses.

The dates for live online training are:

3rd to 5th June 2020: 
Validation & Transfer of Methods for Biopharmaceutical Analysis

 

24th to 26th June 2020:
Validation & Transfer of Methods for Pharmaceutical Analysis

 

There is more information on our online training option on the MTS website but please contact us if you have any questions.

We are also working on converting all of our courses to an on-demand online option which will suit learners who prefer a flexible approach to training and want to fit it around their existing commitments.

Friday, 3 January 2020

6 Free HPLC Tools on the Web


There are quite a few free tools on the web to help you use HPLC effectively. I have chosen six tools to share with you in this blog that I think should prove useful, in no particular order of preference. Please let me know if there are any others that you think should have been mentioned and I’ll include them in a future blog.

1. HPLC Troubleshooting Guide by Uwe Neue
This excellent troubleshooting guide by the late Dr. Neue takes the form of a series of questions and answers, where each topic starts off with a question describing a particular problem and the answer suggests a possible solution. This is then followed up with more information and builds up into a dialogue on each troubleshooting topic. The guide is well written in an easy to understand format and is presented as a pdf where the bookmarks have been carefully set up to allow you to navigate through the document easily and find the topic that relates to your troubleshooting enquiry. Click here to access the guide.


2. Troubleshooting Look-up Table
This is a straightforward troubleshooting resource from Macherey-Nagel where you click on your symptom, be it related to chromatography, pressure or a leak, and the webpage will scroll down and present you with a selection of possible reasons for the problem. I think that it is a good resource to prompt a user who has some experience of HPLC about potential sources of typical problems. There is a little bit of product placement in this tool, where Macherey-Nagel products are recommended, but it's fairly minimal. Click here to access the webpage. 


3. HPLC Method Transfer Calculator 
Many manufacturers provide scaling calculators so that you can makes changes to HPLC method parameters and still retain similar chromatography. Recently scaling from HPLC to UHPLC and vice versa has become important as the new capabilities of UHPLC are exploited. One of these calculators is from Supelco, it is available online, and also as mobile app for Android and iPhone/iPad. It’s easy to use, you simply input your current method parameters and the change you wish to make to particle size, column length and internal diameter, the tool will provide the new method conditions. It works for isocratic and gradient methods. 
Click here to access the tool.

4. Liquid Chromatography Textbook
Liquid Chromatography (Chrom-Ed Series) by Raymond P. W. Scott, Library 4 Science
This useful textbook contains detailed descriptions of the components of liquid chromatography systems, e.g. pumps, detectors, etc., and is likely to be helpful to enhance understanding of these. It also covers silica and bonded phases together with some case studies.
The book is available as a free Kindle download. Full details are available at: http://www.chromatography-online.org/amazon_free.html


5. USP Column Equivalency Application
For those of you who don’t work in the pharmaceutical Industry, USP is ‘United States Pharmacopeia’. The column comparison tool that they offer is free to access and useful to anyone using HPLC, not just those who work with pharmaceuticals. The tool will allow you to find a column that is equivalent to one which you specify, or may also be used to find a column which has different selectivity, something that can be very useful during method development. Click here to start the application and when you have agreed to the disclaimer, click on ‘Compare Columns’. There are two database options, I recommend the bottom one, or PQRI (Product Quality Research Institute) Database. Select the column you are interested in from the drop down menu and, if you know there are acids and bases in your sample, tick the appropriate boxes. You can also specify a pH. There are two other options, either select ‘View Similar’ or ‘View Different’ as required. A full description of the approach and how the database was created can be accessed on the webpage.

6. MTS HPLC Calculator
And finally, I couldn’t leave out the HPLC calculator provided by yours truly! This free download includes a range of helpful calculations, none of which you couldn’t do yourself, but intended to be a convenient tool for those with a busy workload. It will help with equilibration times, preparation of mobile phase and using scouting gradients for method development. Click here to access the download page.