Thursday 19 May 2011

How to Develop HPLC Methods

This HPLC method development training course, sponsored by Phenomenex, was held on Tuesday and Wednesday of this week, in Crewe and Edinburgh respectively. The aim of the training was to describe a strategy which can be put in place to develop a new method quickly and easily. The feedback from the delegates was very positive. Comments included:
“Excellent course, found it very informative and useful."
"Very enjoyable & informative, Oona was extremely helpful.”
“I enjoyed the exercises as they made you think and apply what we just learnt.”

There are two more dates for this course next week, in London on Tuesday and in Milton Keynes on Wednesday. If you are interested in attending, contact Phenomenex (course code SS0-5947) to check if there are any places left. Tel: UK: 01625 501367 or email:

Course Details:
Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes.
This course is ideal for those who have experience of running HPLC methods and now want to learn how to develop new methods.

Course Outline
Developing an HPLC method using a 5-step strategy:
 Step 1: Setting suitable objectives for method development
 Step 2: Assessing all available information
 Step 3: Selecting suitable samples
 Step 4: Performing scouting experiments to select suitable initial conditions
 Step 5: Optimising the method to define method parameters which achieve the desired separation
This course focuses on reversed phase mode separations.

Practical Skills Acquired
This course will enable you to take a strategic approach to developing HPLC methods with an understanding of the factors which can be adjusted to manipulate the retention time of analytes. In addition you will be able to:
1. Define the objectives for the development of a HPLC analytical method.
2. Effectively assess all the available relevant information relating to the desired method, e.g. pKa of the analyte.
3. Select and prepare a suitable sample or samples to be used for the method development.
4. Select suitable scouting conditions to find a suitable column and mobile phase system.
5. Optimise the chromatographic conditions to result in the best possible separation.

Course price is £195 + VAT per delegate. The price includes: Full day training (including post training assessment), course literature, technical brochures, lunch and refreshments.

A follow up course: ‘How to Develop HPLC Methods for Challenging Separations’ (course code SS0-5946) is planned for September, at Crewe on the 13th and Milton Keynes on the 20th. The aim of this course is to enable development of methods for problem samples and analytes, examples being very polar molecules and samples containing numerous analytes.

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