Thursday, 19 May 2011
There are two more dates for this course next week, in London on Tuesday and in Milton Keynes on Wednesday. If you are interested in attending, contact Phenomenex (course code SS0-5947) to check if there are any places left. Tel: UK: 01625 501367 or email: firstname.lastname@example.org
Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes.
This course is ideal for those who have experience of running HPLC methods and now want to learn how to develop new methods.
Developing an HPLC method using a 5-step strategy:
Step 1: Setting suitable objectives for method development
Step 2: Assessing all available information
Step 3: Selecting suitable samples
Step 4: Performing scouting experiments to select suitable initial conditions
Step 5: Optimising the method to define method parameters which achieve the desired separation
This course focuses on reversed phase mode separations.
Practical Skills Acquired
This course will enable you to take a strategic approach to developing HPLC methods with an understanding of the factors which can be adjusted to manipulate the retention time of analytes. In addition you will be able to:
1. Define the objectives for the development of a HPLC analytical method.
2. Effectively assess all the available relevant information relating to the desired method, e.g. pKa of the analyte.
3. Select and prepare a suitable sample or samples to be used for the method development.
4. Select suitable scouting conditions to find a suitable column and mobile phase system.
5. Optimise the chromatographic conditions to result in the best possible separation.
Course price is £195 + VAT per delegate. The price includes: Full day training (including post training assessment), course literature, technical brochures, lunch and refreshments.
A follow up course: ‘How to Develop HPLC Methods for Challenging Separations’ (course code SS0-5946) is planned for September, at Crewe on the 13th and Milton Keynes on the 20th. The aim of this course is to enable development of methods for problem samples and analytes, examples being very polar molecules and samples containing numerous analytes.