Do you have any problems relating to analytical chemistry for pharmaceuticals or training? Send your questions to the MTS helpdesk using our contact form.
Question:
“I am working with TFA in my mobile phase and my analyte elutes at 13.5 minutes. In the same day the retention time has changed by 0.2 minutes. It is normal? I'm using an isocratic method.”
Answer:
"If you experience a change in retention time during a run then it indicates that something in the method conditions is changing, most likely a temperature or mobile phase pH shift. In my opinion, 0.2 minutes is a very small change for a peak at 13.5 minutes (~1.5%) and may not necessarily be a big problem. However if the shift continues, it may become an issue. I suggest that you check the following:
- Is the column temperature controlled? If not, then that is probably the source of the RT shift and if you have no way to introduce temperature control then you will experience RT shift. If you do have a column oven, then ensure that it is working correctly and that there is a suitable length of tubing to and from the column also being temperature controlled.
- Is your analyte ionisable? TFA is a very useful acid modifier for HPLC mobile phases and is successful for a range of molecules. However it is an acid modifier rather than a buffer and so some ionisable analytes may not be suitably pH controlled using only TFA.
Posts
