If you're looking for a good learning tool that explains the effect of different parameters on an HPLC separation then it might be worth taking a look at HPLC Simulator.
It is described as follows on the developer's website:
"HPLC Simulator
is a web-based high-performance liquid chromatography simulation. Adjust
a range of experimental parameters and see their affect on
chromatographic parameters including retention time, column efficiency,
and backpressure. It is intended to be used for educational purposes
only."
Wednesday 18 September 2013
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