HPLC Calculator is Updated!

The free HPLC Calculator from MTS has been updated following a suggestion from a user. The flow rate has been moved to the gradient table to provide more flexibility.

Please send us any suggestions for improvement or additional calculations that would be helpful.

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20% Discount on All Bookings by LinkedIn Followers!

MTS have a very special offer for you: Any bookings made on or before 14th February 2014 (St. Valentine's day) will be eligible for a 20% discount, provided you are a LinkedIn follower of MTS.

The offer is in addition to existing offers such as early booking discount, group bookings, and multiple course bookings.

All you have to do is click below to follow MTS in LinkedIn.

(Note: you will need to have set up an account in LinkedIn to become a follower.)

Then choose which course or courses you would like to attend. Visit the MTS website for a full list of available courses and dates, or click here for the 2014 calendar. Topics include: Analytical method validation and transfer; stability indicating method development; and basic, intermediate and advanced HPLC.

The offer also applies to our training books:
Validation of Analytical Methods for Pharmaceutical Analysis, and
Introduction to HPLC for Pharmaceutical Analysis.


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MTS Recommends... Calibration of instruments: Is Your UV Spectrometer Accurate Enough

Calibration of instruments: Is Your UV Spectrometer Accurate Enough 
by Chris Burgess, in Pharmaceutical Technology, Jan 2, 2014

This article takes a statistical look at the calibration requirements for a UV spectrometer.

Training Courses on Method Validation and Transfer in Dublin - March 2014

The first events in the MTS open enrolment training course schedule for 2014 will be held in Dublin on the 26th to 28th March.

The courses on offer are:
Validation of Analytical Methods for Pharmaceutical Analysis, 26th & 27th March - early booking rate is €1050 + VAT
Transfer of Analytical Methods for Pharmaceutical Analysis, 28th March - early booking rate is €610 + VAT

Check out the Course List page on the MTS website for more information on these training courses, including detailed course descriptions. More details about costs and available discounts for multiple course bookings and groups can be found on the booking forms (also accessed from the course list page).


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Help on: How long should it take to purge the lines on my HPLC system?

MTS HELPDESK
Do you have any problems relating to analytical chemistry for pharmaceuticals or training? Send your questions to the MTS helpdesk using our contact form.

Question:
"When I am setting up the HPLC systems in the laboratory where I work, the relevant procedure directs that the new mobile phase is flushed through to the purge valve on the pump for 5 minutes at a flow rate of 5mL/min. Is this enough? How do you work out the most suitable purge for an HPLC system?"

Answer:
"When setting up an HPLC system, the aim of the purge is simply to flush through all the lines so that any remaining solvent in them from a previous analysis or wash is replaced with the new mobile phase. As you would expect, the amount of new solvent required depends on the volume of the tubing in the system. The major contribution to the volume of tubing is usually the on-line vacuum degasser, if installed. The design of these modules is such that they require a relatively large volume to perform well. For example, a typical Agilent 1200 degasser has a volume of ~12 mL for each line. Agilent recommends a purge of at least 30mL in total for the 1200 systems.

Therefore, to estimate how much volume is required for a purge, check the volume specification of the inlet tubing, the degasser and the pump that you are using, then double it to give an approximate purge volume. You may find that the manufacturer of your particular instrument has a recommended amount to use. A flow rate of 5mL/min is commonly used for standard HPLC systems.

If you have a mixture of different brands of HPLC instruments (and degassers) within one laboratory and want the procedure to cover them all then find out which requires the longest purge and use that as the default."

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